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. 2019 Dec 5;10(12):930. doi: 10.1038/s41419-019-2171-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Relative position and construction of reporter vectors of ZNF667-AS1. The pGL3-control vector was used as a positive control (POS), and empty pGL3-basic vector as a negative control (EV). b Relative position and construction of reporter vectors of ZNF667. POS: pGL3-control vector, EV: pGL3-basic vector. c Luciferase activity of unmethylated and methylated pGL3-A1, pGL3-Z1, and pGL3-Z2 reporter constructs in Eca109 cells. d Binding sites of transcription factor Sp1 and E2F1 in the promoter region of ZNF667-AS1, and luciferase activity of pGL3-A1 and pGL3-A1-mut (binding sites mutation) reporter constructs in Eca109 cells. e Binding sites of transcription factor Sp1 and E2F1 in the promoter region of ZNF667, and luciferase activity of pGL3-Z2 and pGL3-Z2-mut (binding sites mutation) reporter constructs in Eca109 cells. f Binding effect of site 2 or site 5 of ZNF667-AS1 with Sp1 or E2F1 in Eca109 cells. g Luciferase activity of different reporter constructs in Eca109 cells. *P < 0.05. h Relative expression of ZNF667-AS1 in 5-Aza-dC treated or E2F1 transfected Eca109 and TE13 cells. i Relative expression of ZNF667 in 5-Aza-dC treated or E2F1 transfected Eca109 and TE13 cells. The experiments are representative of three independent experiments with similar results. *P < 0.05.