FIGURE 3.

Melanization rate of G. mellonella larvae infected with LD₅₀ dose of MDR-EAEC strains (10⁶ CFU) and treated with MIC dose of Indolicidin 3 h post-infection. MDR-EAEC induced infection (10 μL) was treated with MIC of Indolicidin (10 μL), keeping respective controls (infected, infected with meropenem-treatment, AMP control, PBS control). Data expressed as the mean ± standard deviation of three independent experiments by absorbance monitored at 450 nm. Statistical analysis of melanization rate was performed using the two-way (repeated measures) ANOVA with Bonferroni multiple comparison post-test. ^∗∗∗P < 0.001, ^∗∗P < 0.01, ^∗P < 0.05.