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. 2019 Nov 29;10:2723. doi: 10.3389/fmicb.2019.02723

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Copyright © 2019 Vergis, Malik, Pathak, Kumar, Ramanjaneya, Kurkure, Barbuddhe and Rawool.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LDH cytotoxicity assay of G. mellonella larvae infected with LD₅₀ dose of MDR-EAEC strains (10⁶ CFU) and treated with MIC dose of Indolicidin 3 h post-infection. MDR-EAEC induced infection (10 μL) was treated with MIC of Indolicidin (10 μL), keeping respective controls (infected, infected with meropenem-treatment, AMP control, PBS control). Data expressed as the cytotoxicity (%) of larval hemolymph of three independent experiments. Statistical analysis of LDH cytotoxicity assay was performed using the two-way (repeated measures) ANOVA with Bonferroni multiple comparison post-test. ^∗∗∗P < 0.001, ^∗P < 0.05.