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. 2019 Dec 5;9:18448. doi: 10.1038/s41598-019-54942-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Forward SHG (left, (a,c)) and backward SHG (right, (b,d)) from two menisci of equine adults (A1 for (a,b) and A4 for (c,d)) knee joints. Both menisci show different patterns in forward and backward directions: the backward images better highlights the thick structures, mostly identified as fibers. The images of the same samples (a,b or c,d) are displayed using the same look-up table, but the backward images have been multiplied by a factor indicated in yellow because less signal is physically detected in this direction compared to forward. Scale-bars: 200 μm.