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. 2019 Dec 5;9:18448. doi: 10.1038/s41598-019-54942-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantitative results of F/B and P-SHG. Forward over backward ratio (F/B) over the whole image (a) or for the inter-fibrillar area (b) for all the foetus and adult menisci. (c) Relative inter-fibrillar area measured in backward SHG, i.e. the percentage of inter-fibrillar area compared to the whole image. (d) Circular standard deviation (std) of the P-SHG fibril orientation in the image plane (ϕ): the adult (in dark blue) have values around 32°, whereas the foetuses (in red) split in two groups: one having a value of ϕ circular std rather low (~26°), the other one a larger value (~37°). In each graph, the mean of the different values was taken, and the error bars indicate the standard error of the mean (SEM) of the serie. The statistical significance (NS: non-significant, p < 0.05: significant) was tested using the non-parametric Wilcoxon-Mann-Whitney test. The set of samples consist of n = 5 for foetuses and n = 6 for adults, which gives an acceptable statistical representation given the difficulty to obtain foetus samples.