Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 5;9:18448. doi: 10.1038/s41598-019-54942-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Study design. The meniscus was removed from the equine knee femorotibial stifle (joint) and its body was sectioned at 90° (a). The triangular-shaped coronal (circumferential) sections were deposited on a microscope slide. (b) HEPS stain for standard histology and (c) Picro-sirius red stain for collagen. The rectangle in (b) shows the central area of the tissue investigated in SHG and P-SHG microscopy. (d) Schematic view of the set-up used for P-SHG and F/B SHG. A femtosecond laser directly excites the sample without being scanned, the image being done by moving the sample laterally with a motorized stage, synchronized to the acquisition by a multifunction card (DAQ). QWP: Quarter-wave plate, HWP: Half-wave plate. In P-SHG, the dichroic mirror can be removed to ensure a better polarization control, as the signal is captured in the forward direction. For F/B measurements, a dichroic filter is inserted before the excitation objective to send the backward SHG to a 2^nd detector (II).