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. 2019 Dec 5;9:18393. doi: 10.1038/s41598-019-54846-z

Figure 5.

Figure 5

Suppression of cytokine production by YU102 in LPS-stimulated BV-2 cells. (a) Cytokine and chemokine protein array blots of BV-2 cells treated with vehicle, LPS (1 μg/mL) alone, and YU102 (3 μM) or ONX0914 (3 μM) with LPS (1 μg/mL). Arrow labels indicate cytokines that are most significantly impacted by YU102. Graph depicts the fold change of each cytokine or chemokine (mean). The signal intensity of each cytokine or chemokine was expressed relative to the mean of the intensity of the corresponding spots from vehicle control sample. (b) Cytokine production in LPS-stimulated BV-2 cells with and without YU102 was determined by ELISA. Supernatants of BV-2 cells from 12-well culture plates were used to measure quantities of released cytokines. BV-2 cells were incubated with LPS (1 μg/mL) and YU102 or YU102 epimer for 24 h. All values are expressed as mean ± SEM from three independent experiments. *Differences in suppression of IL-1α and IL-6 levels between YU102-treated and YU102 epi-treated group were statistically significant (p-value < 0.05, n = 3). (c) ELISA-based quantification of serum IL-1α levels (5-fold diluted) in Tg2576 mice. Serum IL-1α levels were significantly lower in YU102-treated Tg2576 mice than in vehicle controls (p-value < 0.05, n = 3). Statistical analysis of ELISA results was performed via Student’s t-test.