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. 2019 Dec 5;10:5549. doi: 10.1038/s41467-019-13479-6

Fig. 1. Establishment and characterization of Meta3 and Meta4 organoid lines.

Fig. 1

a H&E stained slides show stomach mucosa used to isolate metaplastic or dysplastic glands only from the corpus area of Mist1-Kras mouse stomachs at 3 or 4 (Meta3 or Meta4) months after tamoxifen injection. Dotted boxes indicate enlarged area. Scale bars indicate 100 μm. b Phase contrast images of Meta3 and Meta4 organoids captured 0, 1, and 7 days after plating in Matrigel. Red arrows indicate budding structures. Scale bars indicate 50 μm. c Phase contrast images of Meta3 and Meta4 captured at 2 or 4 weeks in 3D culture. Scale bars indicate 50 μm. d Paraffin embedded sections from Meta3 or Meta4 organoids at 4 weeks in 3D culture examined by H&E staining. Red boxes indicate enlarged area. Red arrow indicates a representative multilayered area of Meta4. Scale bars indicate 100 μm. e Quantitation of the average budding rate in Meta3 and Meta4 at 1 or 2 weeks in 3D cultures. Source data are provided as a Source Data file. Data are presented as mean values with standard deviation (n = 9). P-values were calculated using unpaired two-tailed t-test. **P = 0.004; ****P < 0.0001.