Figure 6.

(A) Results from the ThT fluorescence experiments. (A) Group A-T1DM, (B) Group B-T2DM, (C) Group C-Obesity, (D) Group D-Healthy children. Representative sample from every group was analyzed at two different concentrations, and in absence of synthetic monomeric hIAPP. A blank sample was included for comparison. All measurements were performed with a ThT-buffer at 37 °C (PBS 1X, pH 7.4 + 20 μM ThT); protein concentrations of 0.039 mg/mL or 0.0039 mg/mL (B) Comparison of the average t-lag for the trapping experiment (three measurements). One sample from every group was added to synthetic monomeric hIAPP at the same concentration 1:1 (0.039 mg/mL each one), and at one tenth of the concentration 1:0.1 (0.039 mg/mL synthetic hIAPP: 0.0039 mg/mL patient PTS). Synthetic Monomeric hIAPP in absence of patient PTS is included as reference.