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. 2019 Dec 5;9:18465. doi: 10.1038/s41598-019-54570-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Results from the SEC fractionation experiments on a BioSuite 250, 5 μm HR SEC column. The panels on the left show the chromatography for selected representative PTS from each group, both with and without Gn-HCl as denaturant. The panels on the right show the results for all the PTS run for each group, where we can compare the differences between each sample’s peak area and elution time (without Gn-HCl). (A) Group A-T1DM, (B) Group B-T2DM, (C) Group C-Obesity, (D) Group D-Healthy children. All measurements were performed with 0.8 mg/mL of patient PTS or 0.4 mg/mL of patient PTS:Gn-HCl (3 M), in 50 mM Tris-HCl + 50 mM KCl pH = 7.4 at flow rate of 1 ml/min.