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. 2019 Dec 6;4:56. doi: 10.1038/s41392-019-0094-1

Fig. 7. NR4A1 signals DNA-PKcs to interact with p53 and is involved in the development of ARLD.

Fig. 7

a–d Proteins were obtained from hepatocytes, and NR4A1 siRNA was used to inhibit NR4A1 expression. Then, the expression of p-DNA-PKcs, p53 and NR4A1 was measured. e Co-IP was used to detect the interaction between DNA-PKcs and Ku80. f The protein interaction between DNA-PKcs and p53. g–j The expression of the mitophagy markers phosphorylated FUNDC1, mito-LC3II and Beclin1 in response to NR4A1 knockdown were measured by western blotting. k, l Mitochondrial fission was observed, and the mitochondrial length was measured. m–p Paraffin-embedded liver sections were stained with hematoxylin and eosin. Hepatic fibrosis was detected by Sirius Red staining. Frozen liver sections were subjected to Oil Red O staining. q Caspase-9 activity was applied to investigate cellular damage. The experiments were repeated three times with similar results. The data represent the mean ± standard error of the mean. *p < 0.05.