FIGURE 6.

Tyrosine hydroxylase levels are abnormally high in the dopamine neurons of flies fed a high-salt diet. (A) DA and OA synthesis pathways in Drosophila. (B) qPCR analysis of DA and OA synthesis pathway-related genes in the heads of control flies and flies fed a diet supplemented with 1% NaCl. Three independent biological samples were conducted. (C) Representative image of the central brain of a control fly stained with anti-TH. The positions of the four major DA cell clusters are indicated. The white outline shows the brain. Scale bar: 100 μm. (D–G) Images of PPL1, PPL2, PPM3, and PPM12 cell clusters stained with anti-TH from a control fly and a fly fed with HSD. Scale bars: 10 μm. (H) Quantification of TH intensity in the dopamine neurons (n = 8–10 per group). Values plotted are means ± S.D.; ^∗∗P < 0.0, ^∗∗∗P < 0.001 relative to levels in the control.