FIGURE 7.

Reduction of the rate-limiting enzyme in DA synthesis or silencing of DA neurons blocks the effects of HSD on sleep changes. (A–D) TH-KO^Gal4 heterozygous mutant flies (n = 60–80 per group) fed with or without 1% NaCl were monitored, and the following were determined for a 24 h period, in daytime, and in nighttime: (A) minutes of sleep per 30-minute period, (B) average sleep duration, (C) number of sleep episodes, and (D) mean sleep episode duration. (E) Schematic of the UAS-shi^ts transgene driven by TH-Gal4 used to silence DA neurons. (F–I) TH-Gal4 > UAS-shi^ts flies and control flies (n = 40–60 per group) were reared at 18°C, and were then transferred to 29°C and fed a sucrose/agar diet or a diet supplemented with 1% NaCl. The following were determined for a 24 h period, in daytime, and in nighttime: (F) minutes of sleep per 30-minute period, (G) average sleep duration, (H) number of sleep episodes, and (I) mean sleep episode duration. Values plotted are means ± S.D.; ^∗P < 0.05.