Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 6;4:58. doi: 10.1038/s41392-019-0091-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — MKP-1 reduced APP processing in N2A^APP cells. a–e The protein levels of APP b, C89 c, C99 d, and BACE1 e, assessed by western blot in lysates of N2A^APP cells after overexpression of MKP-1 by LV_MKP-1 or knockdown of MKP-1 by LV_shMKP-1. *p < 0.05 by one-way ANOVA. n = 5–6 in each group. f, g The generation of Aβ₄₀ f and Aβ₄₂ g as measured by ELISA in the conditioned media of N2A^APP cells after overexpression of MKP-1 by LV_MKP-1 or knockdown of MKP-1 by LV_shMKP-1. *p < 0.05 by one-way ANOVA. n = 5–6 in each group. h, i The mRNA levels of APP h and BACE1 i as assessed by qPCR in N2A^APP cells after LV_MKP-1 transfection. *p = 0.017 for APP and *p = 0.038 for BACE1 by unpaired Student’s t test. n = 3–4 in each group. j, k The promoter activity of APP j and BACE1 k assessed by luciferase assay in cells transfected with LV_MKP-1. ***p < 0.001 and **p = 0.004 by unpaired Student’s t test. n = 3–4 in each group. l, m The degradation of APP l and BACE1 m assessed by half-life measurements in LV_MKP-1-transfected N2A^APP cells treated with 100 μg/ml cycloheximide (CHX). p = 0.438 for APP and p = 0.483 for BACE1 by two-way ANOVA. n = 4–5 in each group.