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. 2019 Aug 8;47(16):8632–8648. doi: 10.1093/nar/gkz677

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Identity of the first and last nucleotide of the repeat affect efficiency and specificity during spacer integration. (A) Annotation of repeat sequence mutation of guanine at position 1 and position 36. (B) Integration reaction with mutated CRISPR targets taken at time points: 15 s, 1 min and 15 min. (C) High-throughput sequencing analysis of integration products represented as percent of total reads mapped throughout the linear CRISPR target. Guanine at position 1 and 36 of the repeat are displayed as G1 and G36. Nucleotide level resolution of high-throughput sequencing data is provided in Supplemental Figure S4. Range of total number of reads (6,114–16,093).