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. 2019 Aug 8;47(16):8632–8648. doi: 10.1093/nar/gkz677

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — Ruler-based mechanism influences second-site integration in vivo. (A) (left panel) Annotated sequence of expanded CRISPR array in vivo with single and double insertion and deletion mutations in the repeat sequence. (Right panel) Sites of integration represented as percent total of mapped reads at the leader-repeat junction (LR) and spacer–repeat junction (SR). (B) (left panel) Sequences spanning the repeat–spacer junction and site of integration. Red line highlights expected site of integration relative to insertions and deletions upstream and downstream of the ruler element (5′-CTGTA-3′) as indicated by red arrows or boxes. (Right panel) Integration site at the positions spanning the repeat–spacer junction.