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. 2019 Aug 8;47(16):8649–8661. doi: 10.1093/nar/gkz679

Figure 1.

Figure 1.

Detection of viroids in the purified ribosomes of the total ribosome fraction of infected plants. (A) The symptoms of tomato cv Rutgers infected with CEVd at 3 wpi as compared to those of mock-inoculated (Control) tomato plants. (B) The symptoms of tomato cv Rutgers infected with PSTVd-RG at 3 wpi as compared to those in mock-inoculated (Control) tomato plants. (C) Northern blot hybridization analysis of the isolated total ribosomes of both CEVd (upper panel) and PSTVd-RG (lower panel) infected tomato plants (Inf) and their corresponding control plants (Cont). 5S rRNA was used as a control. (D) RT-PCR of the total RNAs from control (C) and CEVd-infected (I) tomato leaves, or of the RNAs from MicroSpin S-400 purified ribosomes from control (CR) and CEVd-infected (IR) tomato leaves. The RT reactions were performed with either the CEVdRT primer (upper panel) or the 25Srev primer (lower panel). The PCRs were performed with either the primers CEVddir and CEVdrev (upper panel) or 25Sdir and 25Srev (lower panel).