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. 2019 Jun 19;47(16):e93. doi: 10.1093/nar/gkz535

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Total RNA libraries enable assembly of single cell transcriptomes. (A) Transcripts were filtered at a length of 200 nt. The remaining transcripts have a mean length of 537 nt. (B) Transcripts were required to have at least two exons. The remaining transcripts are on average 3.4 exons long. (C) All novel genes were quantified on subsampled data (1, 4 or 8 million single-end reads per cell). Genes with at least one count were retained. (D) While some novel genes are expressed in all cells, most novel genes are detected in only one cell.