Skip to main content
. 2019 Jun 25;47(16):8502–8520. doi: 10.1093/nar/gkz545

Figure 4.

Figure 4.

DNA damage enhances the interaction between MORC2 and PARP1 and MORC2 PARylation. (A and B) Lysates from MCF-7 cells treated with or without 1 mM MMS for 1 h were immunoprecipitated with control IgG or an anti-MORC2 (A) or an anti-PARP1 (B) antibody, followed by immunoblotting analysis with the indicated antibodies. (C) MCF-7 cells were treated with or without 1 mM MMS for 1 h and then subjected to immunoblotting analysis with the indicated antibodies. (D-F) MCF-7 cells were pretreated with or without 5 μM Olaparib for 1 h and then treated with or without 1 μM MMS for another 1 h. Lysates were subjected to immunoblotting analysis (D) or IP assays with control IgG or an anti-MORC2 (E) or an anti-PARP1 (F) antibody. The interaction between MORC2 and PARP1 was detected by immunoblotting analysis with indicated antibodies (E and F). (G) MCF-7 cells were treated with DMSO or 1 mM MMS for 30 min. Lysates were subjected to IP and immunoblotting analysis with the indicated antibodies. (H) MCF-7 cells were treated with or without 1 mM H2O2 for the indicate times, and then subjected to IP and immunoblotting analysis with the indicted antibodies. (I) MCF-7 cells were treated with or without 1 μM CPT for the indicate times, and then subjected to IP and immunoblotting analysis with the indicted antibodies.