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. 2019 Jul 5;47(16):8452–8469. doi: 10.1093/nar/gkz567

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 7. — Genetic interactions of Asp1 and Aps1 with the Pol2 CTD impact phosphate homeostasis. (A–C) Strains with the indicated genotypes were grown in liquid culture at 30°C and assayed for acid phosphatase activity. (A) The results show that de-repression of Pho1 by CTD-S7A requires the IPP kinase activity of Asp1. (B) The results show that CTD Thr4 is necessary for de-repression of Pho1 by the asp1 pyrophosphatase-dead H397A allele. (C) The results show that: (i) CTD Thr4 is necessary for de-repression of Pho1 by aps1Δ; and (ii) simultaneous inactivation of Asp1 and Aps1 pyrophosphatases overrides the hyper-repression of Pho1 by CTD-T4A. (D) Strains with the indicated genotypes were spot tested for growth on YES agar at the temperatures specified.