Table 1.

Collection of five high-throughput saturated mutagenesis studies used for analysis

Identifier	Number of variants	Sequence length	Number of variants per residue	Ratio of the residues with variants	PMID
LacI	4041	329	12.28	1.00	8046748
Lysozyme	2015	164	12.29	0.99	1942069
TP53	2314	393	5.89	1.00	27328919
APH(3′)-II	4961	264	18.79	1.00	24914046
BLAT	5198	286	18.17	1.00	24567513

The number of variants per site was calculated by dividing the number of variants by sequence length.