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Extrapolating the New Integration Model to RLE LINES that generate target site duplications. A target site is diagrammed with the first- and second-strand DNA cleavages staggered such that a target site duplication (tsd) would occur upon element insertion. The steps are as in R2 integration, except that the template jump displaces/melts the DNA between the two cleavages to generate the open 4-way junction and the tsd upon DNA synthesis.
