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. 2019 Jun 20;47(16):8399–8409. doi: 10.1093/nar/gkz547

Figure 2.

Figure 2.

H4G interferes with the formation of nucleosome. (A) SDS-PAGE of the canonical histone mixture and the H4G mixture for nucleosome loading. Asterisk (*) represents the GST-H4 and GST-H4G proteins. (B) MNase treatment assay for the GST-H4 and the GST-H4G nucleosomes without DNA extraction. (C) MNase treatment assay for the GST-H4 and the GST-H4G nucleosomes with DNA extraction. 147 represents 147 bp PCR amplified DNA. (D) SDS-PAGE of reconstituted nucleoprotein complexes.