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The α-helix 3 of H4G is important for the nucleolar localization. (A–H) The cellular localizations of the H4G/H4 hybrid constructs in MCF7 cells. Each image is representative of (A) H4sN: N = 131 (99%), (B) H4GsN: N = 160 (88%), (C) H4sC: N = 105 (100%), (D) H4GsN+C: N = 123 (100%), (E) H4sα1: N = 207 (97%), (F) H4sα2: N = 208 (96%), (G) H4sα3: N = 126 (31%) and (H) H4Gsα3: N = 132 (100%). The scale bars represent 10 μm.
