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. 2019 Aug 8;47(16):8913–8925. doi: 10.1093/nar/gkz666

Figure 5.

Figure 5.

The TetR-MphR system was modified to improve the dynamic range of gene expression upon induction. (A) Three copies of the TetR-MphR operator were incorporated into the promoter for controlling GFP expression. (B) Cells containing this modified expression system responded to erythromycin dose-dependently to reach above 8-fold of induction at 1 mM. Each data point represents mean ± S.D. of six biological replicates. Representative raw data of these experiments, acquired with a flow cytometer, are illustrated in panel (C).