Table 1.
Characterization of mutants derived from the TM52 hybrid regulator
| GFP fluorescence levels | |||
|---|---|---|---|
| Mutant | 0 mM Erythromycin | 1 mM Erythromycin | Fold-change |
| TM52 L14T | 24931 ± 199 | 28179 ± 539 | 1.13 ± 0.02 |
| TM52 R54A | 191 ± 14 | 209 ± 18 | 1.09 ± 0.09 |
| TM52 M55L | 1071 ± 32 | 8423 ± 190 | 7.87 ± 0.29 |
| TM52 R54A/M55L | 196 ± 64 | 217 ± 36 | 1.19 ± 0.37 |
| TM52 L14T/R54A | 25497 ± 507 | 27712 ± 467 | 1.10 ± 0.04 |
| TM52 L14T/M55L | 25077 ± 577 | 28210 ± 416 | 1.12 ± 0.03 |
| TM52 (no mutations) | 390 ± 5 | 3299 ± 92 | 8.90 ± 0.19 |
Each data point represents mean ± S.D. of six biological replicates.