Fig. 5.

The cytoplasmic (Red arrows) and nuclear expression (White arrows) of AhR in free LFD and niosomal LFD-treated cells; translocation of AhR was shown in both treatments. The fluorescent signal of the isotype control (secondary antibody) was compensated in quantitative analysis. DAPI-stained nuclei (Blue) were merged with green-florescent emission of AhR-positive cells (40X magnification) (a). Image J was utilized for data quantification in each combination (b). NC: negative control (non-treated cells). Each bar demonstrated the mean ± SD (standard deviation) from three experiments. One-way ANNOVA with Tukey post-test was carried out to compare more than two groups. P-values greater than 0.05 presumed to be not significant. ***P < 0.001