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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: Addict Biol. 2019 Jun 6;25(4):e12779. doi: 10.1111/adb.12779

Figure 8. Inhibition of serotonergic neurons with Kir2.1 blunts the effect of a high yeast diet on ethanol sedation, nutrient consumption and internal ethanol levels.

Figure 8

Male flies of the indicated genotypes consumed 2Y or 30Y media for 1 d prior to determination of ST50s, nutrient consumption, and internal ethanol. (A) There were overall effects of yeast concentration and genotype on ST50s, and an interaction between the two factors (two-way ANOVA; yeast, p<0.0001; genotype, p<0.0001; interaction, p<0.0001; n=8). Compared to flies fed 2Y medium, ST50s were greater in control flies (Trh-Gal4.long3/+ and UAS-Kir2.1/+) on 30Y (*BMC, p<0.0001), but not in flies with inhibition of serotonergic neurons (Trh-Gal4.long3/+; UAS-Kir2.1/+; hatched bars; BMC, p=0.3174). (B) Overall, yeast concentration and genotype influenced nutrient consumption and there was an interaction between yeast and genotype (two-way ANOVA; yeast, p<0.0001; genotype, p<0.0001; interaction, p<0.0001; n=8). All genotypes consumed more nutrients from 30Y than 2Y (*BMC, p≤<0.001). (C) Overall, the concentration of dietary yeast and genotype influenced internal ethanol levels after exposure to vapor from 85% ethanol for 36 minutes (two-way ANOVA; yeast, p<0.0001; genotype, p=0.0072; interaction, p=0.0733; n=8). Internal ethanol was decreased in control flies (Trh-Gal4.long3/+ and UAS-Kir2.1/+) fed 30Y versus 2Y media (*BMC, p≤0.0094), but yeast concentration had no effect on internal ethanol in Trh-Gal4.long3/+; UAS-Kir2.1/+ flies (hatched bars; BMC, p>0.9999).