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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Neuroendocrinology. 2019 Jun 6;110(3-4):271–281. doi: 10.1159/000501337

Figure 3.

Figure 3

PACAP mimics molecular actions of leptin in the VMN. (Top) Western blot image (left) and its graphical representation (right) from VMN punches 30 minutes post PACAP treatment (50pmol/0.25μl/side) show significant PACAP-induced stimulation of phosphorylated STAT3 (pSTAT3) compared to vehicle. Pretreatment with PACAP6–38 (500pmol/0.25μl/side) reversed PACAP-induced STAT3 phosphorylation. In VMN punches, (bottom, left) BDNF and (bottom, right) SOCS3 mRNA were elevated following microinjection of PACAP or leptin. These increases were blocked with pretreatment with PACAP6–38. Data are presented ± SEM. * = P < 0.05