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. Author manuscript; available in PMC: 2020 Dec 4.
Published in final edited form as: Neuron. 2019 Oct 17;104(5):960–971.e7. doi: 10.1016/j.neuron.2019.08.042

Figure 6. TREK-1 and TRAAK channels at nodes of Ranvier of myelinated afferent nerves are required for high-speed saltatory conduction and for sensory behavioral responses.

Figure 6.

A) Left, experimental setting. Right, traces illustrate APs recorded at NRs of shScramble and shTREK-1 groups. Arrow indicates stimulation marks. B) AP conduction velocity (CV) in shScramble (n = 6), shTREK-1 (n = 6), shTRAAK (n = 6), and shTREK-1+shTRAAK groups (n = 6). C) CV in the absence (control, n = 6), present of 5 mM Ba2+ (n = 7), 50 μm NF (n = 6), and 2 μm RR (n = 6). D) CV (n = 7) at NRs at 35°C, 24°C, and 15°C. E) CV at NRs at 15°C without (n = 8), and with 10 μm BL (n = 7), 20 μm AA (n = 6), and intracellular pH of 5 ([pH]i 5, n = 6). AA and [pH]i 5 were applied intracellularly via recording electrodes. F) CV recorded at NRs in the absence (control, n = 8) and presence (n = 8) of voltage-gated K+ channel blockers TEA (20 mM, extracellular), Cs+(135 mM, intracellular), plus 4-AP (1 mM, intracellular). G) Diagram illustrates whisker tactile test. H) Percentage (left) and scores (right) of whisker tactile responses in animals 30 days after microinjection of shScramble (n = 6) and shTREK-1+shTRAAK (n = 6) into D2 whisker hair follicles. I) Percentage (left) and scores (right) of whisker tactile responses in animals 30 days after intra-nerve microinjection of shScramble (n = 6) and shTREK-1 + shTRAAK (n = 6) into D2 whisker nerve branches. Data represent Mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA with the Tukey post hoc test or Student’s t-Test.