(A) Schematic of the NXF1-NXT1 RNA export pathway. The components identified from the screens were colored with red, the gradient of which is proportional to the enrichment significance (casTLE score).
(B) HeLa Flp-In monocistronic reporter cells were induced to express translation reporters by doxycycline after two days of siRNA transfection, and luciferase activities were measured after another 24 hours. NLuc signals were normalized to FLuc in each sample and the relative expression was compared to non-targeting siRNA control. *P<0.05, **P<0.01, ***P<0.001, two-tailed t-test. Data are mean ± s.e.m. from three biological replicates.
See also Figure S1.