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. Author manuscript; available in PMC: 2020 May 20.
Published in final edited form as: Nature. 2019 Nov 20;576(7785):138–142. doi: 10.1038/s41586-019-1774-2

Fig. 3 |. Pgrmc2 regulates heme-sensitive transcription and mitochondrial function in BAT.

Fig. 3 |

a, Total heme (WT, n = 6; PATKO, n = 8) and b, iron (n = 8) levels in BAT. c, Labile heme in mitochondrial and nuclear fractions of BAT (WT n = 5; PATKO n = 8). d, REV-ERBα and BACH1 levels in BAT. e, Genes related to heme and iron metabolism (red portions) are enriched in differentially-expressed genes. f, REV-ERBα and BACH1/2-binding motifs are enriched in genes downregulated in PATKO BAT. g, Heat map of heme and iron related genes shows a global decrease of ETC and TCA gene expression. h, UCP1 and OXPHOS proteins are reduced in PATKO BAT. i, Electron microscopy shows altered mitochondrial morphology in PATKO BAT. Representative images from 4 biologically independent samples. j, Oxygen consumption rate (OCR) of mitochondria isolated from BAT (n = 6). a-j, Biologically independent samples. Representative results from two (a-c, j) or three (d, h) independent experiments. Data presented as mean ± s.e.m. *p<0.05, **p<0.01, ***p<0.001 vs. WT determined by two-tailed Student’s t-test.