Figure 2.

Quantitative proteomics suggests the involvement of cell cycle control in action mechanisms of benzethonium chloride in cancer cells. A. Experimental process of the identification of BZN-regulated proteins by SILAC-based quantitative proteomics experiment. B. Venn diagram showing the identification of overlapped proteins in three biological replicates. C. Differentially expressed proteins with fold change ≥ 1.5 and P ≤ 0.05 in the BZN-treated A549 cells. D. The top five canonical pathways from the IPA analysis of the differentially expressed proteins in BZN-treated cells. E. A549 and H1299 cells were treated with BZN (up to 20 μM) for 48 h, and cell cycle distribution was analyzed by flow cytometry. Note that BZN arrested cells at G1 phase. Bars, SD; *P < 0.05; **P < 0.01; ***P < 0.001 compared with DMSO-treated cells.