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. 2019 Nov 1;9(11):2349–2363.

Figure 5.

Figure 5

AMPK pathway is involved in HSF1-mediated metabolic phenotype switch in OXA-resistance HCC cells. (A) Corresponding network by IPA analysis based on the different expression genes of HCC cells and HCC-OXR cells indicated AMPK pathway is a critical node during the forming of resistance to OXA. Various subunits including α1, α2, β1, β2 and γ (B, C) and p-AMPKα/AMPKα ratio (D-F) in HCC and HCC-OXR cells were detected in AG and LG condition by using western blot. *P < 0.05. Moreover, p-AMPKα/AMPKα ratio was further detected in HSF1-knockdown parental HCC and shHSF1-HCC cells with OXA treatment in AG and LG condition (F, G). *P < 0.05 vs shHSF1-HCC and #P < 0.05 vs shHSF1-HCC (MHCC97H: 6 cycles; Hep3B: 5 cycles) in AG condition; &P < 0.05 vs shHSF1-HCC (MHCC97H: 6 cycles; Hep3B: 5 cycles) in LG condition.