Figure 1.

Suppressing ZEB1 inhibits EC cell metastasis and chemoresistance. A. Scratch migration assay indicated that transfection of shZEB1 into EC cells for 48 h resulted in an impaired migration capacity, being compared to the negative control group. Scale bar: 200 µm. B. Down-regulating ZEB1 stably reduced the migration ability of EC cells in vitro by transwell assay. Scale bar: 250 µm. C. Stably suppressed ZEB1 reduced in vitro invasiveness of EC cells by boyden assay. Scale bar: 250 µm. D. External fluorescence images of lungs of mice were obtained 2 months after tail vein respectively. E. The number of lung metastatic nodules in each group. Scale bar: 5 mm. F. H&E staining of lung metastatic nodules from different experimental groups. Scale bar: 1 mm. G. Dose-response curves of Ishikawa and HEC-1B treated by shZEB1 and PLVctr respectively following 48 h treatment with DDP. The data are expressed as mean ± sd. of three independent experiments. *P < 0.05; **P < 0.01.