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. 2019 Nov 1;9(11):2314–2330.

Figure 3.

Figure 3

ZEB1 interacts with HDGF. A. Co-IP detected the interaction of exogenous ZEB1 and HDGF in EC cells. B. Reciprocal Co-IP of endogenous proteins showed a strong interaction between ZEB1 and HDGF. C. EC cells were immunostained with anti-HDGF (594; red), anti-ZEB1 (488; green) antibody and visualized. Scale bar: 20 µm. D. The transcriptional levels of the HDGF gene were shown after transfecting with ZEB1 plasmid or si-RNA (si-NC, si-ZEB1). ARF was used as a loading control. E. Western blotting showed HDGF protein expression levels after treated with ZEB1 plasmid or si-RNA (si-NC, si-ZEB1) in EC cells. The data are expressed as mean ± s.d. of three independent experiments. *P < 0.05; **P < 0.01.