TABLE 2.

Antimicrobial, hemolytic, and enzymatic activities of wild type (WT), ICMP11096glad-I and ICMP11096glad-IR mutants of B. gladioli pv. agaricicola.

Studied strains	Antimicrobial effecta		Erycrocyte cell lysisb	Extracellular hydrolytic enzymesc
	B. meg	E. col		Protease	Cellulase	Chitinase	Amylase	Glucanase	Pectinase	Polygalacturonase
Bga WT	35 ± 1.5	30 ± 2.7	15 ± 2.1	12 ± 1.3	–	25 ± 3.6	–	28 ± 3.5	–	–
Bga gladI	8 ± 0.7	5 ± 0.2	–	5 ± 0.4	–	5 ± 0.7	–	12 ± 2.1	–	–
Bga gladIR complemented	33 ± 2.4	28 ± 3.3	12 ± 1.5	9 ± 1.9	–	12 ± 1.7	–	22 ± 2.8	–	–

^aAntimicrobial effect of wild type (WT) and mutants of Bga ICMP11096 strain against Bacillus megaterium (B. meg) and E. coli (E. col) expressed as antagonistic capacity percentage; ^bHemolytic effect against erythrocyte cell membrane of fresh bovine blood; ^cExtracellular hydrolytic enzymes (hydrolysis zones in mm) caused by WT, ICMP11096glad-I and ICMP11096glad-IR mutants.