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. 2019 Nov 15;11(11):6838–6849.

Figure 2.

Figure 2

Silencing has_circRNA_403658 inhibited bladder cell growth and invasion. (A) qRT-PCR analysis for has_circRNA_403658 in bladder cancer cell lines, including ccc-HB2, 5637, J82, T24, SW780 and RT4. (B) qRT-PCR analysis for has_circRNA_403658 in 5637 and T24 cells after has_circRNA_403658 siRNA 1# and siRNA 2# transfection. (C, D) Brdu assay was performed to measure the proliferation rate of 5637 (C) and T24 (D) cells after has_circRNA_403658 siRNA transfection. (E, F) Apoptosis analysis for 5637 (E) and T24 (F) cells after has_circRNA_403658 siRNA transfection. (G, H) Transwell assay for 5637 (G) and T24 (H) cells after has_circRNA_403658 siRNA transfection. (I) T24 cells transfected with has_circRNA_403658 siRNA were injected into the back of BALB/c nude mice. The tumor volumes were measured weekly. (J) Immunohistochemistry analysis for Ki67 and c-PARP in xenografted tumor tissues (left), and quantification by imageJ (right). scan bar, 200 μm. *P<0.05 vs control.