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. 2019 Nov 15;11(11):7166–7185.

Figure 6.

Figure 6

Both APC11 and UBA52 required for degradation of CCNB1. (A) Overexpression of UBA52 reduced the level of protein CCNB1 in CCB1 overexpressed A549 cell. (B) Overexpression of UBA52 facilitated cell cycle progression in CCNB1 overexpressed cells, which showed cell cycle arrest in G2/M without manipulation of UBA52 as shown in Figure 2A. (C) As features in cell cycle, overexpression of UBA52 increased the ability of cloning formation of A549 with overexpression of CCNB1. In the circumstance with both UBA52 and CCNB1 overexpressed in non-small cell lines, inhibiting APC11 by small interference RNA resulted in relatively higher level of CCNB1 protein (D), cell cycle arrest in G2/M (E) and less cloning formation (F); in contrast, overexpression of APC11 led to lower level of CCNB1 protein (G), smooth cell cycle progression (H) and more cloning formation (I). ov: overexpression. siRNA: small interfering RNA. *: P<0.05. **: P<0.001.