Table 1.
RT-PCR primers
| Gene name | Forward (5’>3’) | Reverse (5’>3’) |
|---|---|---|
| Bax | CAGTTGAAGTTGCCATCAGC | CAGTTGAAGTTACCATCAGC |
| Bcl-2 | GACTGAGTACCTGAACCGGCATC | CTGAGCAGCGTCTTCAGAGACA |
| Caspase3 | TGGAACAAATGGACCTGTTGACC | AGGACTCAAATTCTGTTGCCACC |
| Sirt1 | CCAGATCCTCAAGCCATG | TTGGATTCCTGCAACCTG |
| SOD1 | GGTGAACCAGTTGTGTTGTC | CCGTCCTTTCCAGCAGTC |
| SOD2 | CAGACCTGCCTTACGACTATGG | CTCGGTGGCGTTGAGATTGTT |
| GPX1 | ATCATATGTGTGCTGCTCGGCTAGC | TACTCGAGGGCACAGCTGGGCCCTTGAG |
| GPX3 | AGAGCCGGGGACAAGAGAA | ATTTGCCAGCATACTGCTTGA |
| IL-1β | GCAACTGTTCCTGAACTCAACT | ATCTTTTGGGGTCCGTCAACT |
| IL-6 | TAGTCCTTCCTACCCCAATTTCC | TTGGTCCTTAGCCACTCCTTC |
| TNF-α | CCTCTCTCTAATCAGCCCTCTG | GAGGACCTGGGAGTAGATGAG |
| IκKα | GTCAGGACCGTGTTCTCAAGG | GCTTCTTTGATGTTACTGAGGGC |
| IκB-α | GGATCTAGCAGCTACGTACG | TTAGGACCTGACGTAACACG |
| P65 | ACTGCCGGGATGGCTACTAT | TCTGGATTCGCTGGCTAATGG |
| GAPDH | ACAACTTTGGTATCGTGGAAGG | GCCATCACGCCACAGTTTC |
RT-PCR, quantitative reverse-transcription polymerase chain reaction.