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. 2019 Nov 15;11(11):7035–7048.

Figure 2.

Figure 2

Knockdown of hnRNP-F could inhibit cell proliferation and cell cycle progression in BC cells in vitro (*P<0.05, **P<0.01 and ***P<0.001). (A) The transfection of sh-hnRNP-F was performed to establish EJ and UMUC-3 cells with the stable knockdown of hnRNP-F expression. HnRNP-F levels were detected in EJ and UMUC-3 cells after hnRNP-F knockdown by western blotting. MTT assay (B) and colony formation assay (C) were performed to detect the effect of hnRNP-F knockdown on the cell proliferation of EJ and UMUC-3 cells. (D) Immunocytochemistry analysis of KI67 protein was performed in EJ and UMUC-3 cells. (E) Cell cycle distribution of EJ and UMUC-3 cells was analyzed by flow cytometry.