Fig. 1.

VK3 and VK3-OH inhibit cell proliferation of neuroblastoma cells.

(A) After treatment with VK3 or VK3 derivative (VK3-OH) for 48 h, IC₅₀ values were determined by WST-8 cell proliferation assay. Each plot showed neuroblastoma-derived cells, and the horizontal line indicated the median values of IC₅₀.

(B) After treatment with VK3-OH for 24 h, cell cycle distributions were determined by flow cytometry. The representative histograms showed the cell cycle distributions of IMR32 cells (top). Each column indicated the cell ratio (bottom). Data represented the mean ± SD of three independent experiments (*P < 0.05, **P < 0.01). DMSO was used as a control.