Fig. 3.

VK3-OH potentiates p53-mediated apoptotic cell death of neuroblastoma cells.

(A) IMR32 cells were treated with the indicated concentrations of VK3 or with VK3-OH. Twenty-four hours after treatment, cell lysates were prepared and analyzed for cleaved caspase-3 and PARP by Western blot analysis.

(B) IMR32 cells were treated with VK3-OH (10 μM). At the indicated time periods after treatment, cell lysates were prepared and analyzed for p53 and p53-related gene products by Western blot analysis.

(C) After treatment with VK3-OH (10 μM) for 24 h, MYCN-inducible SHEP21N cells with or without MYCN overexpression were stained with crystal violet.

(D) Cell cycle distributions of SHEP21N cells with or without MYCN overexpression were determined by flow cytometry.

(E) SHEP21N cells were treated with DMSO or with VK3-OH (10 μM). Six hours after the treatment, cell lysates were prepared and analyzed for the indicated proteins by Western blot analysis. DMSO was used as a control. β-tubulin was used as a loading control.