Figure 4. Downregulation of dPANK/fbl induces decreased viability of adult flies, which is rescued by pantethine and DCA treatment.

• A
  Decreased dPANK/Fbl protein levels were determined in two independent dPANK/fbl‐RNAi strains (1 and 2), in which dPANK/fbl‐RNAi was ubiquitously expressed. A wild‐type strain was used as a control. An anti‐dPANK/Fbl antibody was used to detect protein levels with Western blot analysis on whole fly extracts. Tubulin was used as a loading control.
• B
  Survival rate of adult flies was determined for dPANK/fbl‐RNAi flies, compared to control flies under normal food conditions and after the supplementation of dichloroacetate (DCA) or pantetheine to the food. The assay is explained and visualized in Appendix Fig S3. dPANK/fbl‐RNAi flies show a decreased survival rate compared to control flies. DCA and pantetheine when added to the food increased viability of dPANK/fbl‐RNAi flies. Total number of flies used for each group is indicated above each bar. Statistical analyses were carried out using Fisher's exact test.

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