Skip to main content
. 2019 Nov 11;11(12):e10923. doi: 10.15252/emmm.201910923

Figure 5. TNFα‐CSG treatment reduces tumour stiffness.

Figure 5

Stiffness analyses of the 4T1 tumours following treatment with 4 × daily i.v. injections of indicated compounds. Tumour stiffness was analysed on day 5.
  • A
    En face optical coherence tomography (OCT) images of tumours at a depth of ˜100 μm. Scale bars: 1 mm.
  • B
    The corresponding en face quantitative micro‐elastograms showing tumour elasticity. Scale bars: 1 mm.
  • C
    Plots of stiffness distribution in the tumours.
  • D
    Top: Stiffness variance (see Materials and Methods). Bottom: Fractions of pixels between 100 and 1,000 kPa. For both plots, mean ± SEM is shown (n = 3; *P < 0.05 by Student's t‐test).
  • E
    Collagen IV staining (purple = collagen) of whole tissue sections. Scale bars: 1 mm and 100 μm. Selected regions (x and y in B and E) were compared for collagen IV staining at higher magnification (E) and the corresponding micro‐elastograms (B).
Source data are available online for this figure.