Figure EV2. Circ‐CUX1 facilitates CUX1 transcription and aerobic glycolysis of tumor cells.

• A
  Real‐time qRT–PCR assay indicating expression of circ‐CUX1 (normalized to β‐actin) in IMR32, SH‐SY5Y, LoVo, or PC‐3 cells stably transfected as indicated (n = 5). One‐way ANOVA, *P < 0.05 versus empty vector (mock) or scramble shRNA (sh‐Scb).
• B
  Dual‐luciferase assay showing the activity of CUX1 promoter in IMR32, SH‐SY5Y, LoVo, or PC‐3 cells stably transfected as indicated (n = 5). One‐way ANOVA, *P < 0.05 versus mock or sh‐Scb.
• C
  Real‐time qRT–PCR assay indicating expression of CUX1 isoforms (normalized to β‐actin) in IMR32 and SH‐SY5Y cells stably transfected as indicated (n = 5). One‐way ANOVA, *P < 0.05 versus empty vector (mock) or scramble shRNA (sh‐Scb).
• D
  Real‐time qRT–PCR assay indicating CUX1 mRNA levels in IMR32 and SH‐SY5Y cells stably transfected as indicated (n = 5), and those treated with actinomycin D (5 μg ml⁻¹). One‐way ANOVA, NS: non‐significant.
• E
  Seahorse extracellular flux assay showing ECAR and OCR in IMR32, SH‐SY5Y, LoVo, or PC‐3 cells stably transfected as indicated (n = 6). One‐way ANOVA, *P < 0.05 versus mock or sh‐Scb.

Data information: Data are presented as mean ± SEM. Exact P values are specified in Appendix Table S4.