Table 1. Summary of primary antibodies used in this study.
| Antibody | Cell phenotype | Specificity | Isotype | Source |
|---|---|---|---|---|
| pAb A 0452 | T cells | Human CD3 | Rabbit immune serum | DAKOd) |
| mAb BAQ15A | B cells, FDCs | Bovine CD21 | IgM | VMRDe) |
| mAb BAQ44A | B cells | Undefineda) | IgM | VMRDe) |
| mAb CNA.42 | FDCs | 120-kd FDC specific glycosylated antigen [30] | IgM | DAKOd) |
| mAb 2–137 | FDCs | Undefinedb) | IgM | A. Youngc) |
| mAb F99/97.6.1 | N/A | PrP | IgG1 | VMRDe) |
a) Exact epitope undefined. Antibody developed using bovine PBMCs as antigen. Staining characteristic determined to specifically label a portion of B cells in peripheral blood by FACS profile available at www.vmrd.com. b) Exact epitope undefined. Antibody developed using membrane suspension of sheep ileal Peyer’s patches as antigen [7]. Specifically labels the surface and cytoplasm of cells within the GC that are morphologically consistent with follicular dendritic cells (FDCs). C) Antibody generously gifted by Dr. Alan Young, South Dakota State University. d) Dako, Glostrup, Denmark. e) VMRD, Pullman, WA, U.S.A.