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. 2019 Oct 31;13(5):803–816. doi: 10.1016/j.stemcr.2019.10.005

Figure 5.

Figure 5

Treatment with Cyclosporine A Results in Improved Mitochondrial Function in hiPSC-ECs

(A–C) The OCR revealed increased mitochondrial function in hiPSC-EC NCRM1 treated with 500nM CsA during differentiation (A).

Both maximal mitochondrial respiration (B) and mitochondrial reserve capacity (C) were increased in hiPSC-ECs treated with CsA (n = 3).

(D) Mitochondrial activity was also tested by MTT after addition of 500 nM CsA or 10 mM MitoTEMPO during differentiation. CsA results in an increased mitochondrial activity, whereas MitoTEMPO did not increase mitochondrial activity.

(E) Fold change of fluorescent intensity per mg of protein of ROS staining shows reduced ROS of CsA-treated cells. The same effect was obtained by addition of 10 mM MitoTEMPO.

(F and G) Mitochondrial DNA measured with qPCR (F) and mitochondrial density quantified on TEM stitches (21 cells/group) (G) after treatment of CsA during differentiation.

Values are presented as mean ± SEM of n = 3–4 independent experiments. One-way ANOVA or non-paired two-tailed Student's t test was performed; ∗p < 0.05, ∗∗p < 0.001, ∗∗∗p < 0.0001; ns, not significant.