Table 2.
Summary of Quantified Properties for NAP1-Loaded Tetrasomes
| Quantity | (H3.1-H4)2 Tetrasomes on DNArandom | (H3.1-H4)2 Tetrasomes on DNAw/601 |
|---|---|---|
| Δz(dis)ass (nm) | 24 (±3)a | 23 (±5)b |
| Δθ(dis)ass (turns) | 0.73 (±0.05)a | 0.9 (±0.2)b |
| Δθflipping (turns) | 1.7 (±0.1)a | 1.6 (±0.2)b |
| τD,left (s) with time average in filtering of 3.3 s [N = 330] | 183 (+15/−12)c [A] | 37 (±2)d [A] |
| 62 (±3)d [B] | ||
| 141 (+11/−10)d [C] | ||
| τD,left (s) with time average in filtering of 19.1 s [N = 1910] | 417 (+60/−48)c [A] | 67 (+5/−4)d [A] |
| 121 (+8/−7)d [B] | ||
| 231 (+24/−20)d [C] | ||
| τD,right (s) with time average in filtering of 3.3 s [N = 330] | 14 (±1) [A] | 28 (+2/−1)d [A] |
| 33 (±1)d [B] | ||
| 30 (±2)d [C] | ||
| τD,right (s) with time average in filtering of 19.1 s [N = 1910] | 34 (+5/−4)d [A] | 48 (+4/−3)d [A] |
| 64 (±4)d [B] | ||
| 44 (+5/−4)d [C] | ||
| τD,left/τD,right with time average in filtering of 3.3 s [N = 330] | 13 (±1)c [A] | 1 (±0)c [A] |
| 2 (±0)c [B] | ||
| 4 (±0)c [C] | ||
| τD,left/τD,right with time average in filtering of 19.1 s [N = 1910] | 12 (±3)c [A] | 1 (±0)c [A] |
| 2 (±0)c [B] | ||
| 5 (±1)c [C] | ||
| ΔEdwell-times (kBT) | 2.6 (±0.1)c [A] | 0.3 (±0.1)c [A] |
| 0.6 (±0.1)c [B] | ||
| 1.5 (±0.1)c [C] | ||
| ΔEpeak areas (kBT) | – | 0.9 (±0.7)c [A] |
| 1.4 (±0.8)c [B] | ||
| 1.7 (±—–) [C] |
Specific buffers employed are indicated within brackets. The results for spontaneously loaded tetrasomes are provided in Table S12.
a
Values taken from (35).
b
Values obtained for spontaneously loaded tetrasomes on DNAw/601 are included for improved statistics.
c
Errors calculated by error propagation.
d
Errors correspond to 68% confidence interval for estimated values from exponential fits.