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. 2019 Nov 2;117(11):2054–2065. doi: 10.1016/j.bpj.2019.10.036

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© 2019 Biophysical Society.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Analysis of nuclear foci by STED-ICCS and object-based localization. Analysis of representative STED images of MCF10A cells acquired upon labeling of (a) BrU (green) and H3K9me3 (red), (b) PCNA (green) and EdU (red), and (c) BrU (green) and EdU (red). Shown are (from left to right) the dual-color STED image, the positions of the colocalized (cyan) and non-colocalized (red and green) foci recovered by particle localization, the map of the colocalized fraction recovered by local ICCS, the RDD histogram, and the spatial correlation functions recovered by ICCS. The ICCS plot shows the cross-correlation function (black triangles) and the red (red squares) and green (green circles) channel autocorrelation functions along with the corresponding fits (solid lines). Scale bars, 4 μm. To see this figure in color, go online.